Fig. 6. Cytoplasmic amphiphysin II translocates into RIN3-positive vesicles in HeLa cells. (A,B) HeLa cells were transiently transfected with GFP-amphiphysin II (A) or GFP-amphiphysin II/SH3 (B) and RFP-mock (A,B, top) or RFP-RIN3 (A,B, bottom) and further incubated for 48 hours. The fluorescence of RFP (left) and GFP (centre) was visualized by confocal microscopy, and merged images of the two signals are displayed in yellow (right). (C) HeLa cells were transiently transfected with RFP-RIN3 (left), GFP-amphiphysin II (middle) and YFP-Rab5 (right), and further incubated for 48 hours. Scale bars, 10 µm.