Fig. 3. Immunolocalisation of GFP-Hrs, GFP-Y329/334F and GFP-UIM. HeLa cells were transfected with GFP-Hrs (A,D), GFPY329/334F (B,E) or GFP-UIM (C,F) and fixed 22 hrs post-transfection with 3% PFA, permeabilised with Triton X-100 and stained with either anti-transferrin receptor (TrfR, A-C, shown in red) or anti-mannose-6-phosphate receptor (Man6PR, D-F, shown in red) antibodies followed by secondary antibodies coupled to Alexa Fuor 594. All panels show a single confocal section of a group of cells presenting the most typical staining pattern for each construct. Insets show a threefold enlargement of selected areas. Scale bars: 20 µm.