Fig. 5. PGs1-GFP localises to the centrosome. (A,B) 1009 cells were transfected with a PGs1-GFP construct and analysed 24 hours after transfection. Cells were fixed with paraformaldehyde and stained with L83 (right panel in A) or CTR-453 (right panel in B). Left panels show PGs1-GFP fluorescence. (C,D), PGs1-GFP fluorescence of stable transfected 1009 cells, fixed with paraformaldehyde (C) or unfixed (D). Scale bars: 10 µm. (E) Western blotting of 1009 (lane 1) and stable transfected 1009/PGs1-GFP (lane 2) cell extracts with L83. A single band at 60x10³ was detected in the latter but not in the former. Note that the endogenous PGs1 is not detected.