Fig. 7. 3D reconstruction of PGs1 at the centrosome. 1009 EC cells were fixed with methanol and double stained with L83 (red) and GT335 (A, green) or GTU-88 (B, green) or CTR-453 (C, green) or DM1A (D, green). Confocal images were analysed by deconvolution. Projections of all planes are shown in left panels. 3D reconstructions are shown in the middle and right panels in two different orientations. In D (right), the green colour was made semi-transparent to better visualise the red staining. The arrowheads in D (left), indicate PGs1 dots associated with MTs.