Fig. 2. Nuclear localization of p120-3A is not dependent on its classical nuclear localization sequences. (A) p120 structure. Multiple isoforms of p120 exist due to the use of alternative ATG start sites and alternative splicing of exons A, B, and C (CC, coiled-coil domain; PD, phosphorylation domain). p120 has two consensus NLS sequences, one localized in the phosphorylation domain and the other in the Arm repeat 6. (B) Deletion mutants were expressed by retroviral transduction in cadherin-deficient A431D cells and their distribution examined by immunofluorescence. p120-3A localizes in the nucleus (i), but deletion of each NLS separately (ii and iii) or in combination (iv) does not affect nuclear localization of p120-3A.