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Fig. 4. PtK1 cells immunostained for Mad2 (red) and {gamma}-tubulin (green). The overlay of phase contrast images, Mad2, and {gamma}-tubulin staining is shown. (A) Cell arrested in mitosis by a 3-hour nocodazole treatment. (B-D) Cells fixed after 1, 5 and 15 minutes recovery from the nocodazole-induced mitotic arrest. Scale bars: 10 µm.