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Fig. 5. PtK1 cells immunostained for Mad2 and {alpha}-tubulin. The {alpha}-tubulin staining is shown in the left column and the overlay of phase contrast images and Mad2 immunostaining is shown in the right column. (A,A'). Cell fixed after a 3 hour nocodazole treatment. (B,B'-D,D'. Cells fixed after 1, 5 and 15 minutes recovery from the nocodazole-induced mitotic arrest. Scale bar: 10 µm.