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Fig. 9. Frequencies of merotelic orientations in late prometaphase/metaphase PtK1 cells and in `delayed' metaphases (see text for details). (A) Frequencies of merotelic orientations in prometaphase/metaphase PtK1 cells (-MG-132), and metaphases `delayed' by a 2 hour MG-132 treatment (+MG-132). Both in control (CTRL) and nocodazole-recovering (NOC) cells, the frequency of merotelic orientations after a 2-hour MG-132 treatment was lower than the frequency before the MG-132 treatment. N, total number of cells analyzed. (B) Frequencies of single and multiple merotelic kinetochore orientations in PtK1 cells recovering from nocodazole, with or without MG-132 treatment.