Fig. 7. Co-localization of Syt IX and tubulin. RBL-Syt IX⁺ cells were: left untreated (A-D); treated for 30 minutes with 5 µM taxol (E-H); allowed to internalize FITC-conjugated Tfn (50 µg/ml) for 30 minutes, followed by 30 minutes of treatment with taxol (I); or transiently transfected with Rab 11-GFP cDNA and treated for 30 minutes with taxol (J). Cells were labeled with rabbit anti-C2A-Syt IX (A-H) or mouse anti-ß-tubulin (A-J), followed by rhodamine/FITC-conjugated donkey anti-mouse IgG or rhodamine/FITC-conjugated donkey anti-rabbit IgG as indicated. D and H are the phase-contrast images of A-C and E-G, respectively. Bars, 3 µm (A-H); 2 µm (I,J).