Fig. 8. Movement of intracellular organelles within the leading process of migrating neurons cultured on a glial cell layer. (A) The leading process of an inhibitory neuron (asterisk) moved beneath a process of an adjacent neuron (arrowheads). The images were collected at 10 minute intervals. The nucleus of the migrating neuron was not able to pass beneath the adjacent process; by contrast, cytoplasmic components moved into the leading process towards the growth cone, resulting in swelling (arrow) of the middle portion of the leading process. Bar, 20 µm. (B,C) Additional examples of neurons with a swelling (arrows) of the leading process. Immunostaining revealed the presence of the Golgi apparatus and the centrosome (arrowheads) within the swellings. Right-hand panels are merged images of staining with anti-pericentrin (green), anti-Golgi 58K protein (red) and DAPI (blue). Bar, 10 µm.