Fig. 1. Immunofluorescence of stressed cells and western blot analysis of protein extracts. (A) Unstressed (Control) cells, cells heat shocked (+HS) at 42°C for 60 minutes, cells treated with cadmium 50µM (+Cd) for 2 hours and cells transfected with E1A (E1A) for 12 hours, were fixed and immunolabeled for PML. Some microstructures are indicated by arrows. (B) Unstressed cells (–HS) and cells that were heat shocked at 43°C for 30 minutes (+HS) were immunolabeled for PML and HSP70. Note the increased levels of HSP70 and the presence of microstructures in cells that were heat shocked. (C) Western blot analysis of protein extracts made from control (–HS) cells and cells heat shocked at 43°C for 30 minutes (+HS). Arrow indicates the specific bands corresponding to HSP70. Lower band is of non-specific activity, indicating that the increase in HSP70 is not due to unequal loading. Full image widths of micrographs correspond to 15 µm.