Fig. 2. A role of dynein in the budding yeast S. cerevisiae. Dynein mediates attachment of astral microtubules (AMT; purple lines) to the bud cortex and sliding of the microtubules on the attachment site (red arrow). Consequently, the nucleus (blue sphere) moves into the bud neck (black arrow). Dynein also drives cortical sliding of microtubules in the mother cell (red arrow), generating an opposing force on the nucleus. As a result, the nucleus remains at the neck with oscillations between the mother and bud cells (black arrows). The opposing forces also orient the mitotic spindle along the cell axis, and contribute to spindle pole separation.