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Fig. 6. Ct-eIF4H is not bound to nuclear poly(A+) RNA. C. tentans tissue culture cells were or were not UV-irradiated and poly(A+) RNA was isolated from nuclear extracts. The co-purified proteins were analyzed by western blotting. Ct-eIF4H did not co-purify with poly(A+) RNA after UV-cross-linking (lanes 1 and 2), whereas hrp36 did (lanes 3 and 4).