Fig. 7. The Bpag1 ABD, but not the isoform 2 N-terminus, localizes to focal contacts. (A,B) As observed by confocal microscopy, the FLAG-ABD fusion protein staining (green) consistently produced staining that overlapped with paxillin staining (red), indicating its localization to focal contact sites. (C,D) With the FLAG-Nterm2 fusion protein (green), only minor overlap with paxillin staining (red) was ever observed by confocal microscopy. Also, punctate staining consistently observed for paxillin and FLAG-ABD along the periphery of the cells (arrows in A and B) was never observed with the FLAG-Nterm2-expressing cells. Bar in D, 10 µm, and applies to all four images.