Fig. 2. CD82 does not affect ligand-independent dimerization of EGFR and ErbB2. Cells were pre-treated with 0.5 mM BS3 for 1 hour at 4°C and then subjected to immunoprecipitation analysis as described in Materials and Methods. Immunoprecipitated proteins were resolved in 6% SDS-PAGE, transferred to the nitrocellulose membrane and probed with anti-EGFR (mAb-15) or anti-ErbB2 (mAb-1) Abs.