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Fig. 7. CD82 causes redistribution of GD1a in MCF-7 cells. Cells were prepared for immunofluorescence staining as described in the legend to Fig. 6. (A) MCF-7/ZEO cells were stained with anti-GD1a mAb. Staining was visualised using FITC-conjugated goat anti-mouse IgG. (B,C) Double staining of MCF-7/CD82 cells was carried out using a combination of anti-GD1a (GD1a-1) and anti-CD82 ({gamma}C12) mAbs (B and C, respectively). Staining was visualised using a combination of Texas Red-conjugated goat anti-IgG1 and Alexa Fluor 488-conjugated goat anti-IgG2a. (D) Digital superimposition of B and C.