Fig. 3. Reduced trabecular and cortical bone in junB^–/– Ubi-junB animals. (A) Bone sections of 4-week-old (top), 8-week-old (middle) and 16-week-old (bottom) control and mutant littermates were stained with Haematoxylin and Eosin, magnification was 10x (top and middle) or 5x (bottom). (B) TRAP staining of osteoclasts in distal tibiae sections of 4-week-old control and junB^–/– Ubi-junB littermates. (C) In situ detection of MMP-9-expressing osteoclasts and chondroclasts in proximal tibia sections of 4-week-old control (middle) and mutant mice (right). Hybridisation of control section with sense probe served as control for specificity (left). Sections were counterstained with Haematoxylin and Eosin; magnification was 20x (B,C).