Fig. 1. Genetic mosaics of selD^ptuf in the eye. (A) selD^ptuf clones were recovered to adulthood and resulted in aberrant eyes. The mutant area (light red sectors; arrowheads) was scarred and almost no ommatidia differentiated. (B) Tangential sections of selD^ptuf clones revealed no ommatidial cells present in the mutant area. Right half: mutant sector; left half: normal tissue. (C) selD^ptuf homozygous clones in the eye imaginal disc stained with the anti-Elav neuronal cell marker (red; left) indicated that differentiation of photoreceptors is altered. In the middle panels mutant clones (black) lack ß-galactosidase staining (green). Right panels: merged images. A confocal section at the normal level of Elav pattern (upper panels) showed no labeling in the selD^ptuf clone; however, deeper in the mutant tissue (lower panels) Elav staining occurs, showing abnormal ommatidia (arrows). (D) TUNEL staining (red) in a selD^ptuf clone of an eye imaginal disc. It shows that cells in the selD^ptuf clone (dark area lacking ß-galactosidase labeling) entered apoptosis. In addition to wild-type apoptosis in the vicinity of the morphogenetic furrow (arrow), ectopic apoptotic figures were observed in cells adjacent to the clone (arrowhead). (E) In vivo detection of ROS with DHE (red) in selD^ptuf clones (dark area lacking GFP labeling) DHE labeled cells were present in the clone and also in cells adjacent to it (arrowhead).