Fig. 3. Differential distribution of FA proteins in FX and FA. Endothelial cells migrating into an in-vitro `wound' were fixed and immunolabeled for PY (left column) and different FA proteins (middle column) as indicated. Analysis of the differential distribution of the different proteins relative to PY was assisted by calculation of pixel-to-pixel ratio images (right column). VASP forms a chain of 1-2 µm dots, located in front of the PY-positive FX while it co-localizes with PY in FA. Paxillin localizes extensively in both FA and FX. Zyxin appears both in a wavy pattern along actin and in FA; however, it is completely absent from FX at the leading edge. Scale bar: 5 µm.