Fig. 6. FX induced by active Rac do not contain zyxin. PAE cells were transfected with dominant active Rac (L61) and double-labeled for PY and zyxin. Transfected cells have a round morphology, and while maintaining some FA they show a substantial accumulation of FX around the cell periphery, as seen by the PY staining. Zyxin is associated with FA and with actin filaments, but is absent from FX. Scale bar: 5 µm; insets are enlargements (3x) of the boxed areas.