Fig. 7. Effect of inhibition of RhoA and Rho kinase on MCP-1-induced changes in brain endothelial permeability. (A) Western blot analysis of TJ proteins (occludin, claudin-5, ZO-1 and ZO-2) after treatment of cell with Rho kinase and RhoA inhibitors, Y27632 and C3 or transiently transfected with dominant negative mutant T19NRho. Effect of Rho and Rho kinase inhibitors on changes (B) TEER and (C) [¹⁴C]inulin permeability (P) induced by MCP-1. Results are presented as means±s.e.m., n=3 independent experiments.