Fig. 6. Effect of a lithogenic diet on keratin expression levels in the gallbladder and liver. (A) Total tissue homogenates of gallbladders (GB) from K8^–/–, K8^+/– and K8^+/+ mice that were fed a normal or lithogenic diet were analyzed by immunoblotting using antibodies to the indicated keratins and to actin. (B) Total tissue homogenates of livers from the same mice used in A were analyzed by immunoblotting using antibodies to actin, tubulin and the indicated keratins. Note that the LD induces overexpression of keratins in WT mouse gallbladder but not liver.