Fig. 7. Effect of a lithogenic diet on keratin phosphorylation in the gallbladder and liver. (A) Liver sections from K8^+/+ (a,b) or K8^–/– (c,d) mice that were fed control diet (a,c) or LD (b,d) were stained by indirect immunofluorescence using anti-K8/18 antibodies. Note the absence of keratins in K8^–/– liver, except for staining of biliary ductal cells owing to the presence of K7 and K19 in these cells. (B,C) Liver (B) and gallbladder (C) sections from K8^+/+ mice that were fed a control or lithogenic diet were triple stained using toto-3 (blue; nuclear DNA staining), anti-K8/K18 or anti-K18-pS33 (red), and anti-K8 pS436 or pS79 (green). L, lumen. Asterisks in b and d of B indicate areas of injury. Note the induction of liver and gallbladder keratin phosphorylation at the three different K8/K18 phosphorylation sites tested, after exposure to the lithogenic diet. Scale bars in A,B,C: 20 µm.