Fig. 6. Activation of Xral in the marginal zone of Xenopus embryos. (A,C) Experimental scheme showing animal cap (AN), marginal zone (MZ), endoderm (EN) and vegetative pole (VG) domains that were dissected (A) or injected with wild-type XralB mRNA (4x 500 pg) (C). Protein from explants (B) or whole, injected embryos (D) were extracted and analysed for RalB-GTP content by pull-down, as described in the Materials and Methods and in Fig. 1.