Fig. 10. Effect of III_1c on IST-9 binding to EDA⁺ fibronectin. Wells were coated with 20 µg/ml of cellular fibronectin (EDA⁺) and then blocked with 1.0% BSA. Triplicate wells were incubated with increasing amounts of III_1c for 1 hour. Following treatments, the binding of EDA specific mAbs, IST-9 (2 µg/ml), or 5C11F3 (8 µg/ml) was determined by ELISA. The graph depicts data from a representative experiment performed in triplicate.