Fig. 5. Effect of III_1c on fibronectin matrix morphology. Fibroblasts were cultured on glass coverslips in the presence of fibronectin conjugated with Alexa Fluor-488 (10 µg/ml), indicated as AF-FN, for three days. Wells were washed and incubated with 20 µM III_1c or carrier buffer as control for 20 hours. Cells were washed, fixed, permeabilized and stained for fibronectin using the designated mAbs. Alexa Fluor-488 labeled fibronectin was visualized and total matrix fibronectin was detected by indirect immunofluorescence using either an antibody against the CS domain (FDB3) or an antibody against the EDA domain (IST-9). The figure represents one experiment repeated three times.