Fig. 8. Kinetics of III_1c-induced loss of IST-9 binding. Fibroblast monolayers, grown as described previously, were incubated with 20 µM III_1c or III_11c for increasing times. The wells treated with carrier buffer were set as the 100% control. After incubation with fibronectin fragments, cell layers were washed, fixed and processed for ELISA using the monoclonal antibody IST-9 (2 µg/ml). Data points are the mean of triplicate wells. The graph depicts data from a representative experiment performed in triplicate.