Fig. 9. Binding of III_1c to EDA⁺ and EDA^– fibronectins. Triplicate wells were coated with 20 µg/ml of either (EDA⁺) cellular fibronectin or (EDA^–) plasma fibronectin, blocked with 1.0% BSA and incubated with increasing amounts of III_1c for 1 hour. Bound III_1c was measured by ELISA using a monoclonal antibody to the 6xHis tag.