Fig. 3. Comparison of GFP fluorescence in intracellular parasites transiently expressing TgSODB2^1-84/GFP, TgSODB2^1-287/GFP, or TgACP/GFP. The apicoplast is delineated as a single, discrete dot of GFP fluorescence in pTgACP/GFP transfected cells (third row, four-cell stage), while parasites transfected with the SOD constructs show a twisting pattern of GFP fluorescence characteristic of the parasite mitochondrion (first and second rows, four- and two-cell stages, respectively). Mock transfected parasites showed no fluorescence when photographed with the same exposure time. Scale bar: 5 µm.