Fig. 4. TgSODB2 localizes to the parasite mitochondrion. Parasites were transiently transfected with pTgSODB2^1-84/GFP and pTgSODB2^1-287/GFP and imaged for GFP and Mitotracker-red^TM fluorescence. (A) Deconvolved images of intracellular parasites (two-cell stage), showing extensive Mitotracker^TM staining of host mitochondria, as well as colocalization of GFP and Mitotracker^TM in the parasite mitochondrion. (B) Extracellular parasites, showing colocalization of GFP and Mitotracker^TM. Scale bar, 5 µm.