Fig. 5. TgSODB2 is imported into the mitochondrion. A western blot of lysate from parasites stably expressing TgSODB2^1-287/GFP shows two bands (grey arrows) in addition to the endogenous TgSODB2^1-287 and TgSODB2^85-287 bands (black arrows) seen in 2F lysate: a faint band at about 60 kDa corresponding in size to TgSODB2^1-287/GFP and a much more abundant product at about 50 kDa corresponding to TgSODB2^85-287/GFP. mrSODB2 recognized all four bands, whereas GFP recognized only the 50 and 60 kDa, GFP-containing products. HFF cell lysate is included as a negative control.