Fig. 6. Mutating the N-terminal extension results in mistargeting phenotypes. Parasites transiently transfected with pTgSODB2^26-84/GFP, which contains a deletion of the presequence (PS), showed GFP fluorescence in a punctate pattern within the parasites (four-cell stage). GFP signal was also detected in the PV, where it colocalized with GRA4. Transfection with pTgSODB2^1-25/GFP bearing a deletion of the hydrophobic sequence (HS) resulted in diffuse localization within the parasite cytoplasm in a pattern distinct from the mitochondrion, visualized by Mitotrackerred^TM staining. Transfection with pTgSODB2^R12A/GFP, in which arg¹² was replaced by an ala (asterisk), showed a discrete localization pattern that colocalized with an apicoplast targeted protein, FNR(l)-dsRed. Scale bar, 5 µm.