Fig. 7. The chromosomal passenger protein INCENP is localised normally in Topo II-depleted cells with the exception of a few highly abnormal anaphase cells. (A) Control RNAi-treated normal prometaphase cell. (B) Control RNAi-treated normal telophase cell. (C) Topo II RNAi-treated prometaphase cell with abnormal chromosomal morphology. (D) Topo II RNAi-treated late prometaphase cell with abnormal chromosomal morphology. (E) Topo II RNAi-treated abnormal cell with INCENP stretched along the chromosome arms. (F) Topo II RNAi-treated anaphase cell with massive chromatin bridging, but INCENP located normally on the central spindle. (G) Topo II RNAi-treated late prometaphase cell with abnormal chromosomal morphology: Aurora-B localises normally on the centromeres. (A-G) DAPI staining for DNA; (A'-G') anti-tubulin shows the mitotic spindle; (A"-F") staining for INCENP; (G") staining for Cid shows the positions of the kinetochores; (G'") staining for the chromosomal passenger Aurora B. In A'"-F'" DAPI is blue, tubulin is red, INCENP is green, and in G'''' DAPI is blue, Cid is red, Aurora-B is green. Scale bar: 5 µm.