Fig. 2. Dynactin subunits localize around both female and male pronuclei, whereas dynein distributes only to the female. (A) Dynactin subunits p150^Glued, p50 and p62 distribute around both female (F) and male (M) pronuclei during pronuclear migration and apposition in bovine zygotes. Additional diffuse staining is detected in the cytoplasm for both p150^Glued (far left) and p50 (center left). Pre-incubation of anti-dynactin p150^Glued antibodies with human endothelial cell lysates results in a loss of detection in zygotes (far right). (B) Double-staining of dynein 74 kDa (green) and dynactin p150^Glued (red) shows co-localization around the female, but not the male, pronucleus (Merge; top). Staining of calreticulin (red) shows distribution throughout the cytoplasm but no localization to pronuclear surfaces (Merge; bottom). Scale bars, 10 µm.