Fig. 9. DAP kinase promotes focal adhesion disassembly under serum-stimulated conditions. (A) NIH3T3 cells co-transfected with various DAP kinase constructs and GFP were cultured in serum-containing medium. Two days after transfection, cells were fixed and stained with anti-paxillin. The percentages of GFP-positive cells that display a weaker paxillin-staining pattern than the neighboring GFP-negative cells were quantified and are indicated on the right. The values shown are means ± s.d. from at least three independent experiments. (B) Cells as in (A) were directly analysed by IRM without fixation. (A,B) Transfected cells are visualized by GFP fluorescence and marked with arrows; (A) the phase-contrast image is also included. Scale bars, 10 µm.