Fig. 1. The amino-terminal domain of TPX2 contains a nuclear localization signal and a microtubule-binding site. Fragments of TPX2 were fused to EGFP, expressed in HeLa cells and then fixed 24 hours after transfection. Microtubules were visualized using an anti-tubulin antibody (YOL1/34), DNA was visualized with DAPI, and the whole cells were viewed by differential interference contrast (DIC) microscopy. (A) TPX2 fragments fused to EGFP that localize to the nucleus (TPX2, TNT and 1T) or the cytoplasm (3T and 5T). (B) EGFP-TPX2 fragments that co-localize with microtubules (MT). (C) Summary outlining the sub-cellular localizations of the different fragments of TPX2 (amino acid residues in parenthesis), N, nuclear localization; C, cytosolic localization; box depicts the NLS-containing domain. Scale bar: 10 µm.