Fig. 7. Confocal images at high magnification, of wild-type and targeted (c20) cells co-immunostained for myomesin and telethonin (A-F); skeletal myosin and MURF2 (G-L), or skeletal myosin and obscuring (M-R). The staining for each antibody is shown separately, together with the merged image. Arrow in H indicates microtubular-like staining; arrows in O indicate M-line staining. Both wild-type and c20 cultures contain myotubes at a variety of stages of differentiation as seen here, with the myotubes in the central region typical of the most highly differentiated. Scale bar: 10 µm.