Fig. 6. Hippocalcin(1-14) co-localises with NCS-1 but KChIP1(1-11) does not. Cells were transfected to express hippocalcin(1-14)-EGFP or KChIP1-EYFP along with NCS-1 either as a wild-type or ECFP-tagged construct. In these dual transfected cells the minimal myristoylation sequence from hippocalcin (hippocalcin (1-14)) targeted EGFP (B) to the same sites as NCS-1 detected by immunofluorescence (A) with complete overlap of localisation as shown in yellow in the overlaid image (C). In contrast, KChIP1(1-11)-EYFP (E) showed a distinct localisation to that of NCS-1-ECFP (D,F). The colour overlays show NCS-1 in green and the other constructs in red. Scale bar: 10 µm.