Fig. 4. Changes of mRNA detected on a cDNA Atlas Array. Confluent cells were stimulated without (A) or with biglycan (B) for 24 hours. RNA was extracted, reverse transcribed to cDNA, radioactively labelled and hybridised using the Atlas Human Cell Interaction Array. The whole membranes are shown to the left and the small GTPases, RhoA, Rac and Cdc42, as well as paxillin and zyxin are indicated in the expanded figures.