Fig. 6. Stimulation with biglycan and decorin increases cell migration. Cell migration assays were performed in cell culture inserts with 50,000 cells added to the inserts in medium supplemented with or without 10 ng/ml PDGF-BB. (A-C) Cells were allowed to migrate for 8-24 hours as control (A), or stimulated with 10 µg/ml biglycan (B) or decorin (C) and thereafter fixed and finally stained with Crystal Violet. (D) Cell counting was performed after stimulation with or without PDGF-BB. (E,F) Cells were also counted after stimulation with biglycan, decorin, DS or CS in absence (E) or presence (F) of PDGF-BB. A-C show representative experiments of stimulation for 24 hours in presence of PDGF-BB. Values are given as means±s.e.m. for n=2-5; ^*significant differences of treatment compared to control.