Fig. 6. (A) Western blot analysis on total protein extracts obtained from mice sacrificed 15 minutes, 1 hour or 3 hour after saline, SKF82958, pilocarpine or kainic-acid injection. 15 µg of proteins were separated on SDS/PAGE gels and revealed using anti-P.ERK, anti-panERK (ERK), anti-P.H3 (P), anti-Ac₁₄.H3 (Ac₁₄) and anti-P-Ac₁₄.H3 (P-Ac₁₄) antibodies. (B) Phosphorylation-acetylation of histone H3 on c-fos and MKP-1 chromatin. ChIP assays were performed on chromatin obtained from hippocampi of mice injected with 35 mg kg^–1 kainic acid (GLU) or saline treated (–), using a preimmune serum (no Ab), anti-P.H3 (P), anti-Ac₁₄.H3 (Ac₁₄) or anti-P-Ac₁₄.H3 (P-Ac₁₄). The DNA recovered from the antibody-bound fraction and the DNA from the input chromatin were analysed by PCR with oligonucleotides specific for the indicated gene.