Fig. 6. Expression of adherens junction proteins in E6 cells. (A) Cells grown to low and high cell densities were stained with an anti-E-cadherin MAb. a,a', Neo cells; b,b', Glu18E6 cells; c,c', WT18E6 cells; d,d', Leu18E6 cells. The level of decrease of E-cadherin at peripheral contact sites in E6 cells correlated with the activity of the PDZ-binding motif. (B) Cells were stained with a MAb specific for ß-catenin (a, Neo cells; b, Glu18E6 cells; c, WT18E6 cells; d, Leu18E6 cells. Bar, 20 µm (A and B, a-d) and 40 µm (A, a'-d'). (C) Total extracts of cells grown to 80% confluency were analysed for protein expression levels of adherens junction proteins (E-cadherin, P-cadherin, -catenin and ß-catenin). Note that HeLa cells are E- and P-cadherin negative. Actin levels control for loading.