Fig. 6. Apical expression of moesin is independent of Foxj1 expression in airway epithelial cells. (A) Wild-type (+/+) and Foxj1-null (–/–) MTE cells were cultured and immunostained for expression of ezrin (clone 3C12; green) and moesin (red) at indicated day (d) as in Fig. 2B. Confocal microscopy images were reconstructed to generate z-axis images. Bar, 10 µm. (B) Mouse lung cells were immunostained for moesin (Franck et al., 1993) (red) and ezrin (green). Arrow indicates a rare cell expressing only apical moesin. Bar, 10 µm. (C) Wild-type MTE cells from ALI day 10 (left) or mouse lung cells from wild-type and Foxj1-null mice (right) were immunostained for expression of moesin (red) and CCSP (green). Bar, 10 µm.