Fig. 7. Apical membrane moesin cannot compensate for ezrin in Foxj1-null cells. (A) MTE cells were grown to ALI day 10 as in Fig. 2, then immunostained for expression of either EBP50 (red) or ß₂AR (red) together with ezrin (green). Immunofluorescent microscopy images focused on the apical membrane of cells in identical fields analyzed for expression of ezrin, EBP50, or ß₂AR then merged. Bar, 10 µm. (B) MTE cells from wild-type (+/+) and Foxj1-null (–/–) mice cultured as in A, immunostained for expression of either EBP50 or ß₂AR (both green) together with lateral cell junction membrane protein E-cadherin (E-cad, red) and imaged by confocal microscopy at the level of the apical membrane (x,y). Images were reconstructed to generate z-axis images. Bar, 10 µm. (C) Tissue sections from wild-type and Foxj1-null mouse lungs were immunostained for expression of indicated apical ezrin-dependent and basolateral protein as in Fig. 1. Arrows mark apical expression of ß₂AR. Bar, 10 µm.