Fig. 4. Quantification of FC size and motility under static and flow conditions. (A) The size of FCs in cells was quantified by counting pixels of labeled FCs at 0, 60 and 120 minutes after the initiation of shear stress, in the form of flowing medium at 12 dynes/cm². (B) Movement of FCs was assayed in non-treated (static) cells and in cells subjected to flow over periods 0-60 and 60-120 minutes after the initiation of shear stress. A total of 40 FCs in three cells were evaluated for each time point. ^*Indicates a significant difference (P<0.01).