Fig. 5. The dynamics of vimentin filament and ß3 integrin-rich focal contacts 120-220 minutes after the initiation of flow. Populations of TrHBMECs co-expressing YFP-ß3 integrin and CFP-vimentin was subjected to flowing medium at 12 dynes/cm2 and then the live cells were viewed for 120-220 minutes after the initiation of shear stress. Images of a typical treated cell at 120, 170, and 220 minutes are shown. The YFP images at (A) 120, (B) 170 and (C) 220 minutes were coded in blue, green and red, respectively and then merged (D). The white colors of the large FCs in D indicate that they show minimal movement in the plane of the membrane. Note also that each FC in A-C shows extensive interaction with vimentin IF. Scale bar: 3 µm. This can be viewed in video form (movie 5, http://jcs.biologists.org/supplemental/).