Fig. 9. TrHBMECs treated with vimentin siRNA show reduced adhesion under flow conditions, while those treated with cytochalasin D do not. TrHBMECs were transfected with control siRNA (A,B) or vimentin siRNA (C,D) as indicated and after 72 hours the cells were subjected to flow stress. (E,F) Non-transfected cells were incubated with 0.1 µM cytochalasin D for 30 minutes prior to and during flow treatment. Medium was passed over the cells at 12 dynes/cm² as before. Phase contrast images of the cells are shown at 0 and 50 minutes. Scale bar: (in F) 10 µm. (G) The number of cells attached after flow treatment for 50 minutes. Cells were treated with control siRNA, vimentin siRNA or 0.1 µM cytochalasin D (as above). The data were derived from observations of three separate trials involving at least 20 cells per trial.