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Fig. 1. SDS-PAGE and immunoblot analysis of junctional proteins of the cytoskeletal fractions from bovine lens fiber cells, including cortical regions. Proteins of water-insoluble fractions were separated by 8% PAGE and probed with antibodies to the following proteins: lanes 1 and 2, N-cadherin (N-cad); lanes 3 and 4, cadherin-11 (Cad-11); lanes 5 and 6, E-cadherin (E-cad); lanes 7 and 8, {alpha}-catenin ({alpha}-cat); lanes 9 and 10, ß-catenin (ß-cat); lanes 11 and 12, ezrin (81 kDa); lanes 13 and 14, periplakin (~190 kDa) (Ppl); lanes 15 and 16, vinculin (Vinc). Two lanes are shown for each probe, the left with a lower protein load and the right with a higher protein load. Molecular weights of reference proteins examined in parallel are given on the left margin.