Fig. 5. Immunolocalization of adherens junction proteins in cryosections through near-cortical regions of bovine lens fiber cells. Immunofluorescence microscopy showing the hexagonal appearance of the fiber cells, with lateral long sides and apical short sides, after double immunolabeling with antibodies against N-cadherin (A,B,D, red; N-cad) in combination with either -catenin (A', green; -cat) or cadherin-11 (B', green; Cad-11), with antibodies against cadherin-11 in combination with ß-catenin (C', green; ß-cat), or with a combination of antibodies to N-cadherin and ezrin (D', green) observed by confocal laser scanning microscopy. Notice the far-reaching colocalizations of N-cadherin and -catenin (A'', merged picture), cadherin-11 (B'', merge) and – shown here indirectly – with ß-catenin (C'', merge), whereas N-cadherin localized close to ezrin on the short sides, often indicative of partial colocalization (D-D''; for higher resolution see, however, Fig. 8). Notice also the weak, if not absent, reaction of N-cadherin on the long sides. Scale bars, 10 µm.