Fig. 6. Double-label immunofluorescence microscopy of bovine lens fiber cells stained for different cytoskeletal and junctional proteins. Actin (A, red) shows far-reaching colocalization with ezrin (green, notice the yellow reaction in A', merged picture). Almost the entire cell cortex is also positive for plectin (B, green), shown in contrast to the glycoprotein phakinin (B, red). By contrast, in the plane shown in C-C'', antibodies to N-cadherin (C, red; N-cad) intensely decorate the narrow sides, but seem to be excluded from large parts of the lateral membranes, specifically the punctate reaction sites corresponding to gap junctions, which in turn are positive for protein ZO-1 (C'', merged picture). Scale bar, 10 µm.